ω-Transaminase-Mediated Asymmetric Synthesis of (S)-1-(4-Trifluoromethylphenyl)Ethylamine

The pivotal role played by ω-transaminases (ω-TAs) in the synthesis of chiral amines used as building blocks for drugs and pharmaceuticals is widely recognized. However, bulky are challenging to produce. Herein, a ω-TA (TR8) from marine bacterium was synthesize fluorine amine ketone. An analysis reaction conditions process development showed that isopropylamine concentrations above 75 mM had an inhibitory effect on enzyme. Five different organic solvents were investigated co-solvents ketone (the acceptor), among which 25–30% (v/v) dimethyl sulfoxide (DMSO) produced highest enzyme activity. reached equilibrium after 18 h at 30% conversion. situ product removal (ISPR) approach using aqueous two-phase system tested mitigate inhibition. activity initially decreased because substrate preferentially partitioned into phase, n-hexadecane. Consequently, DMSO added increase mass transfer without affecting ability phase prevent inhibition product. Thus, maintained, amount increased 62 time. can be bioconversion ketones future bioprocess applications.